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hek cells  (ATCC)


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    ATCC hek cells
    DISC1–PML interaction is required for NPC proliferation in the developing cortex. A, lysates from human embryonic kidney <t>(HEK)</t> <t>cells</t> co-transfected with DISC1, PML and WT IE1 or IE1-L174P were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. B, lysates from HEK cells cotransfected with DISC1, IE1, and WT PML or mutant PML lacking the IE1-binding site (PMLΔIE1) were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. C, HEK cells cotransfected with PML and HA-tagged WT DISC1, or mutant DISC1 lacking the PML-binding site (DISC1ΔPML), were immunoprecipitated with the PML antibody and immunoblotted with the HA antibody. D, mouse embryos electroporated with various constructs at E13.5 were pulse labeled with BrdU (50 mg/kg) for 2 h at E15.5. Bar graph represents the percentage of GFP- and BrdU-double positive cells over total GFP-positive cells in the VZ/SVZ. Green , cells transfected with GFP, DISC1 RNAi, and DISC1 constructs; red , BrdU-positive cells; arrowheads indicate GFP- and BrdU-double positive cells. The scale bar represents 20 μm. Graph shows mean +/− SEM (GFP: n = 4, DISC1 RNAi: n = 6, DISC1 RNAi + Wt DISC1: n = 4, DISC1 RNAi + DISC1ΔPML: n = 3, Tukey’s multiple comparison test ∗ p < 0.05; one-way ANOVA: F(3,13) = 6.067, p = 0.0082). BrdU, bromodeoxyuridine; E13.5, embryonic day 13.5; E15.5, embryonic day 15.5; HA, hemagglutinin; NPC, neural progenitor cell; IE1, immediate early 1; SZ, subventricular zone; VZ, ventricular zone; PML, promyelocytic leukemia.
    Hek Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 741 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Cytomegalovirus-encoded immediate early 1 protein perturbs neural progenitor proliferation via interfering with host PML–DISC1 interaction"

    Article Title: Cytomegalovirus-encoded immediate early 1 protein perturbs neural progenitor proliferation via interfering with host PML–DISC1 interaction

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2026.111269

    DISC1–PML interaction is required for NPC proliferation in the developing cortex. A, lysates from human embryonic kidney (HEK) cells co-transfected with DISC1, PML and WT IE1 or IE1-L174P were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. B, lysates from HEK cells cotransfected with DISC1, IE1, and WT PML or mutant PML lacking the IE1-binding site (PMLΔIE1) were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. C, HEK cells cotransfected with PML and HA-tagged WT DISC1, or mutant DISC1 lacking the PML-binding site (DISC1ΔPML), were immunoprecipitated with the PML antibody and immunoblotted with the HA antibody. D, mouse embryos electroporated with various constructs at E13.5 were pulse labeled with BrdU (50 mg/kg) for 2 h at E15.5. Bar graph represents the percentage of GFP- and BrdU-double positive cells over total GFP-positive cells in the VZ/SVZ. Green , cells transfected with GFP, DISC1 RNAi, and DISC1 constructs; red , BrdU-positive cells; arrowheads indicate GFP- and BrdU-double positive cells. The scale bar represents 20 μm. Graph shows mean +/− SEM (GFP: n = 4, DISC1 RNAi: n = 6, DISC1 RNAi + Wt DISC1: n = 4, DISC1 RNAi + DISC1ΔPML: n = 3, Tukey’s multiple comparison test ∗ p < 0.05; one-way ANOVA: F(3,13) = 6.067, p = 0.0082). BrdU, bromodeoxyuridine; E13.5, embryonic day 13.5; E15.5, embryonic day 15.5; HA, hemagglutinin; NPC, neural progenitor cell; IE1, immediate early 1; SZ, subventricular zone; VZ, ventricular zone; PML, promyelocytic leukemia.
    Figure Legend Snippet: DISC1–PML interaction is required for NPC proliferation in the developing cortex. A, lysates from human embryonic kidney (HEK) cells co-transfected with DISC1, PML and WT IE1 or IE1-L174P were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. B, lysates from HEK cells cotransfected with DISC1, IE1, and WT PML or mutant PML lacking the IE1-binding site (PMLΔIE1) were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. C, HEK cells cotransfected with PML and HA-tagged WT DISC1, or mutant DISC1 lacking the PML-binding site (DISC1ΔPML), were immunoprecipitated with the PML antibody and immunoblotted with the HA antibody. D, mouse embryos electroporated with various constructs at E13.5 were pulse labeled with BrdU (50 mg/kg) for 2 h at E15.5. Bar graph represents the percentage of GFP- and BrdU-double positive cells over total GFP-positive cells in the VZ/SVZ. Green , cells transfected with GFP, DISC1 RNAi, and DISC1 constructs; red , BrdU-positive cells; arrowheads indicate GFP- and BrdU-double positive cells. The scale bar represents 20 μm. Graph shows mean +/− SEM (GFP: n = 4, DISC1 RNAi: n = 6, DISC1 RNAi + Wt DISC1: n = 4, DISC1 RNAi + DISC1ΔPML: n = 3, Tukey’s multiple comparison test ∗ p < 0.05; one-way ANOVA: F(3,13) = 6.067, p = 0.0082). BrdU, bromodeoxyuridine; E13.5, embryonic day 13.5; E15.5, embryonic day 15.5; HA, hemagglutinin; NPC, neural progenitor cell; IE1, immediate early 1; SZ, subventricular zone; VZ, ventricular zone; PML, promyelocytic leukemia.

    Techniques Used: Transfection, Immunoprecipitation, Mutagenesis, Binding Assay, Construct, Labeling, Comparison



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    DISC1–PML interaction is required for NPC proliferation in the developing cortex. A, lysates from human embryonic kidney <t>(HEK)</t> <t>cells</t> co-transfected with DISC1, PML and WT IE1 or IE1-L174P were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. B, lysates from HEK cells cotransfected with DISC1, IE1, and WT PML or mutant PML lacking the IE1-binding site (PMLΔIE1) were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. C, HEK cells cotransfected with PML and HA-tagged WT DISC1, or mutant DISC1 lacking the PML-binding site (DISC1ΔPML), were immunoprecipitated with the PML antibody and immunoblotted with the HA antibody. D, mouse embryos electroporated with various constructs at E13.5 were pulse labeled with BrdU (50 mg/kg) for 2 h at E15.5. Bar graph represents the percentage of GFP- and BrdU-double positive cells over total GFP-positive cells in the VZ/SVZ. Green , cells transfected with GFP, DISC1 RNAi, and DISC1 constructs; red , BrdU-positive cells; arrowheads indicate GFP- and BrdU-double positive cells. The scale bar represents 20 μm. Graph shows mean +/− SEM (GFP: n = 4, DISC1 RNAi: n = 6, DISC1 RNAi + Wt DISC1: n = 4, DISC1 RNAi + DISC1ΔPML: n = 3, Tukey’s multiple comparison test ∗ p < 0.05; one-way ANOVA: F(3,13) = 6.067, p = 0.0082). BrdU, bromodeoxyuridine; E13.5, embryonic day 13.5; E15.5, embryonic day 15.5; HA, hemagglutinin; NPC, neural progenitor cell; IE1, immediate early 1; SZ, subventricular zone; VZ, ventricular zone; PML, promyelocytic leukemia.
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    DISC1–PML interaction is required for NPC proliferation in the developing cortex. A, lysates from human embryonic kidney <t>(HEK)</t> <t>cells</t> co-transfected with DISC1, PML and WT IE1 or IE1-L174P were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. B, lysates from HEK cells cotransfected with DISC1, IE1, and WT PML or mutant PML lacking the IE1-binding site (PMLΔIE1) were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. C, HEK cells cotransfected with PML and HA-tagged WT DISC1, or mutant DISC1 lacking the PML-binding site (DISC1ΔPML), were immunoprecipitated with the PML antibody and immunoblotted with the HA antibody. D, mouse embryos electroporated with various constructs at E13.5 were pulse labeled with BrdU (50 mg/kg) for 2 h at E15.5. Bar graph represents the percentage of GFP- and BrdU-double positive cells over total GFP-positive cells in the VZ/SVZ. Green , cells transfected with GFP, DISC1 RNAi, and DISC1 constructs; red , BrdU-positive cells; arrowheads indicate GFP- and BrdU-double positive cells. The scale bar represents 20 μm. Graph shows mean +/− SEM (GFP: n = 4, DISC1 RNAi: n = 6, DISC1 RNAi + Wt DISC1: n = 4, DISC1 RNAi + DISC1ΔPML: n = 3, Tukey’s multiple comparison test ∗ p < 0.05; one-way ANOVA: F(3,13) = 6.067, p = 0.0082). BrdU, bromodeoxyuridine; E13.5, embryonic day 13.5; E15.5, embryonic day 15.5; HA, hemagglutinin; NPC, neural progenitor cell; IE1, immediate early 1; SZ, subventricular zone; VZ, ventricular zone; PML, promyelocytic leukemia.
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    DISC1–PML interaction is required for NPC proliferation in the developing cortex. A, lysates from human embryonic kidney <t>(HEK)</t> <t>cells</t> co-transfected with DISC1, PML and WT IE1 or IE1-L174P were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. B, lysates from HEK cells cotransfected with DISC1, IE1, and WT PML or mutant PML lacking the IE1-binding site (PMLΔIE1) were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. C, HEK cells cotransfected with PML and HA-tagged WT DISC1, or mutant DISC1 lacking the PML-binding site (DISC1ΔPML), were immunoprecipitated with the PML antibody and immunoblotted with the HA antibody. D, mouse embryos electroporated with various constructs at E13.5 were pulse labeled with BrdU (50 mg/kg) for 2 h at E15.5. Bar graph represents the percentage of GFP- and BrdU-double positive cells over total GFP-positive cells in the VZ/SVZ. Green , cells transfected with GFP, DISC1 RNAi, and DISC1 constructs; red , BrdU-positive cells; arrowheads indicate GFP- and BrdU-double positive cells. The scale bar represents 20 μm. Graph shows mean +/− SEM (GFP: n = 4, DISC1 RNAi: n = 6, DISC1 RNAi + Wt DISC1: n = 4, DISC1 RNAi + DISC1ΔPML: n = 3, Tukey’s multiple comparison test ∗ p < 0.05; one-way ANOVA: F(3,13) = 6.067, p = 0.0082). BrdU, bromodeoxyuridine; E13.5, embryonic day 13.5; E15.5, embryonic day 15.5; HA, hemagglutinin; NPC, neural progenitor cell; IE1, immediate early 1; SZ, subventricular zone; VZ, ventricular zone; PML, promyelocytic leukemia.
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    DISC1–PML interaction is required for NPC proliferation in the developing cortex. A, lysates from human embryonic kidney (HEK) cells co-transfected with DISC1, PML and WT IE1 or IE1-L174P were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. B, lysates from HEK cells cotransfected with DISC1, IE1, and WT PML or mutant PML lacking the IE1-binding site (PMLΔIE1) were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. C, HEK cells cotransfected with PML and HA-tagged WT DISC1, or mutant DISC1 lacking the PML-binding site (DISC1ΔPML), were immunoprecipitated with the PML antibody and immunoblotted with the HA antibody. D, mouse embryos electroporated with various constructs at E13.5 were pulse labeled with BrdU (50 mg/kg) for 2 h at E15.5. Bar graph represents the percentage of GFP- and BrdU-double positive cells over total GFP-positive cells in the VZ/SVZ. Green , cells transfected with GFP, DISC1 RNAi, and DISC1 constructs; red , BrdU-positive cells; arrowheads indicate GFP- and BrdU-double positive cells. The scale bar represents 20 μm. Graph shows mean +/− SEM (GFP: n = 4, DISC1 RNAi: n = 6, DISC1 RNAi + Wt DISC1: n = 4, DISC1 RNAi + DISC1ΔPML: n = 3, Tukey’s multiple comparison test ∗ p < 0.05; one-way ANOVA: F(3,13) = 6.067, p = 0.0082). BrdU, bromodeoxyuridine; E13.5, embryonic day 13.5; E15.5, embryonic day 15.5; HA, hemagglutinin; NPC, neural progenitor cell; IE1, immediate early 1; SZ, subventricular zone; VZ, ventricular zone; PML, promyelocytic leukemia.

    Journal: The Journal of Biological Chemistry

    Article Title: Cytomegalovirus-encoded immediate early 1 protein perturbs neural progenitor proliferation via interfering with host PML–DISC1 interaction

    doi: 10.1016/j.jbc.2026.111269

    Figure Lengend Snippet: DISC1–PML interaction is required for NPC proliferation in the developing cortex. A, lysates from human embryonic kidney (HEK) cells co-transfected with DISC1, PML and WT IE1 or IE1-L174P were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. B, lysates from HEK cells cotransfected with DISC1, IE1, and WT PML or mutant PML lacking the IE1-binding site (PMLΔIE1) were immunoprecipitated with an anti-PML antibody and immunoblotted with an anti-HA antibody. C, HEK cells cotransfected with PML and HA-tagged WT DISC1, or mutant DISC1 lacking the PML-binding site (DISC1ΔPML), were immunoprecipitated with the PML antibody and immunoblotted with the HA antibody. D, mouse embryos electroporated with various constructs at E13.5 were pulse labeled with BrdU (50 mg/kg) for 2 h at E15.5. Bar graph represents the percentage of GFP- and BrdU-double positive cells over total GFP-positive cells in the VZ/SVZ. Green , cells transfected with GFP, DISC1 RNAi, and DISC1 constructs; red , BrdU-positive cells; arrowheads indicate GFP- and BrdU-double positive cells. The scale bar represents 20 μm. Graph shows mean +/− SEM (GFP: n = 4, DISC1 RNAi: n = 6, DISC1 RNAi + Wt DISC1: n = 4, DISC1 RNAi + DISC1ΔPML: n = 3, Tukey’s multiple comparison test ∗ p < 0.05; one-way ANOVA: F(3,13) = 6.067, p = 0.0082). BrdU, bromodeoxyuridine; E13.5, embryonic day 13.5; E15.5, embryonic day 15.5; HA, hemagglutinin; NPC, neural progenitor cell; IE1, immediate early 1; SZ, subventricular zone; VZ, ventricular zone; PML, promyelocytic leukemia.

    Article Snippet: SK-N-MC human neuroblastoma cells and HEK cells were purchased from ATCC.

    Techniques: Transfection, Immunoprecipitation, Mutagenesis, Binding Assay, Construct, Labeling, Comparison